p-chk-1 antibody Search Results


chk1 [p ser296] antibody (sn06-50)  (Bio-Techne corporation)
90
Bio-Techne corporation chk1 [p ser296] antibody (sn06-50)
Chk1 [P Ser296] Antibody (Sn06 50), supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/chk1 [p ser296] antibody (sn06-50)/product/Bio-Techne corporation
Average 90 stars, based on 1 article reviews
chk1 [p ser296] antibody (sn06-50) - by Bioz Stars, 2026-03
90/100 stars
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p-chk1 (s345  (Bioworld Antibodies)
90
Bioworld Antibodies p-chk1 (s345
(A) Western blots showing up-regulated level of γH2AX and down-regulated <t>CHK1/2-p53</t> pathway. Level of GAPDH was used as internal controls. Molecular mass is given in kilodaltons. Oocytes were isolated from ovaries of PD35 mice and used for western blot. For each lane, 200 GV oocytes were used. For each experiment, at least 5 mice of each genotype were used. (B) Immunofluorescent staining of 2-month-old ovarian sections showing increased γH2AX in Ppp6c F/F ;GCre+ oocytes. Green: γH2AX; Red: MVH; Blue, DAPI. White arrows point to nucleus of control oocytes; yellow arrows point to nucleus of mutant oocytes. Bar = 20 μm. At least 3 mice of each genotype were used for analysis, and representative images are shown. (C) Decreased incidence of GVBD and PBE of Ppp6c F/F ;GCre+ oocytes. PD35 GV oocytes were isolated and matured in vitro , oocytes that resumed meiosis I (GVBD) and extruded the first polar body (PBE) were counted at 4 h and 13 h, respectively. Data are shown as mean ± SEM. *P< 0.05; **P< 0.01. Representative images of immunostaining for DNA (red) and α-tubulin (green) showing abnormal spindle assembly and aberrant chromosome alignment in Ppp6c F/F ;GCre+ oocytes at 8 h and 13 h, respectively. Bar = 10 μm. In vitro maturation experiments were repeated at least three times.
P Chk1 (S345, supplied by Bioworld Antibodies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/p-chk1 (s345/product/Bioworld Antibodies
Average 90 stars, based on 1 article reviews
p-chk1 (s345 - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
rabbit pab specific for pchk1 phosphorylated on ser345 gtx100065 antibody  (Gentex Corporation)
90
Gentex Corporation rabbit pab specific for pchk1 phosphorylated on ser345 gtx100065 antibody
(A) Western blots showing up-regulated level of γH2AX and down-regulated <t>CHK1/2-p53</t> pathway. Level of GAPDH was used as internal controls. Molecular mass is given in kilodaltons. Oocytes were isolated from ovaries of PD35 mice and used for western blot. For each lane, 200 GV oocytes were used. For each experiment, at least 5 mice of each genotype were used. (B) Immunofluorescent staining of 2-month-old ovarian sections showing increased γH2AX in Ppp6c F/F ;GCre+ oocytes. Green: γH2AX; Red: MVH; Blue, DAPI. White arrows point to nucleus of control oocytes; yellow arrows point to nucleus of mutant oocytes. Bar = 20 μm. At least 3 mice of each genotype were used for analysis, and representative images are shown. (C) Decreased incidence of GVBD and PBE of Ppp6c F/F ;GCre+ oocytes. PD35 GV oocytes were isolated and matured in vitro , oocytes that resumed meiosis I (GVBD) and extruded the first polar body (PBE) were counted at 4 h and 13 h, respectively. Data are shown as mean ± SEM. *P< 0.05; **P< 0.01. Representative images of immunostaining for DNA (red) and α-tubulin (green) showing abnormal spindle assembly and aberrant chromosome alignment in Ppp6c F/F ;GCre+ oocytes at 8 h and 13 h, respectively. Bar = 10 μm. In vitro maturation experiments were repeated at least three times.
Rabbit Pab Specific For Pchk1 Phosphorylated On Ser345 Gtx100065 Antibody, supplied by Gentex Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit pab specific for pchk1 phosphorylated on ser345 gtx100065 antibody/product/Gentex Corporation
Average 90 stars, based on 1 article reviews
rabbit pab specific for pchk1 phosphorylated on ser345 gtx100065 antibody - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
pchk1 s317  (GeneTex)
90
GeneTex pchk1 s317

Pchk1 S317, supplied by GeneTex, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pchk1 s317/product/GeneTex
Average 90 stars, based on 1 article reviews
pchk1 s317 - by Bioz Stars, 2026-03
90/100 stars
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p-chk1(s317) antibody  (Millennium Pharmaceuticals)
90
Millennium Pharmaceuticals p-chk1(s317) antibody

P Chk1(S317) Antibody, supplied by Millennium Pharmaceuticals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/p-chk1(s317) antibody/product/Millennium Pharmaceuticals
Average 90 stars, based on 1 article reviews
p-chk1(s317) antibody - by Bioz Stars, 2026-03
90/100 stars
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phospho-chk1 (p-chk1  (Affinity Biosciences)
90
Affinity Biosciences phospho-chk1 (p-chk1

Phospho Chk1 (P Chk1, supplied by Affinity Biosciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/phospho-chk1 (p-chk1/product/Affinity Biosciences
Average 90 stars, based on 1 article reviews
phospho-chk1 (p-chk1 - by Bioz Stars, 2026-03
90/100 stars
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Image Search Results


(A) Western blots showing up-regulated level of γH2AX and down-regulated CHK1/2-p53 pathway. Level of GAPDH was used as internal controls. Molecular mass is given in kilodaltons. Oocytes were isolated from ovaries of PD35 mice and used for western blot. For each lane, 200 GV oocytes were used. For each experiment, at least 5 mice of each genotype were used. (B) Immunofluorescent staining of 2-month-old ovarian sections showing increased γH2AX in Ppp6c F/F ;GCre+ oocytes. Green: γH2AX; Red: MVH; Blue, DAPI. White arrows point to nucleus of control oocytes; yellow arrows point to nucleus of mutant oocytes. Bar = 20 μm. At least 3 mice of each genotype were used for analysis, and representative images are shown. (C) Decreased incidence of GVBD and PBE of Ppp6c F/F ;GCre+ oocytes. PD35 GV oocytes were isolated and matured in vitro , oocytes that resumed meiosis I (GVBD) and extruded the first polar body (PBE) were counted at 4 h and 13 h, respectively. Data are shown as mean ± SEM. *P< 0.05; **P< 0.01. Representative images of immunostaining for DNA (red) and α-tubulin (green) showing abnormal spindle assembly and aberrant chromosome alignment in Ppp6c F/F ;GCre+ oocytes at 8 h and 13 h, respectively. Bar = 10 μm. In vitro maturation experiments were repeated at least three times.

Journal: PLoS Genetics

Article Title: Protein Phosphatase 6 Protects Prophase I-Arrested Oocytes by Safeguarding Genomic Integrity

doi: 10.1371/journal.pgen.1006513

Figure Lengend Snippet: (A) Western blots showing up-regulated level of γH2AX and down-regulated CHK1/2-p53 pathway. Level of GAPDH was used as internal controls. Molecular mass is given in kilodaltons. Oocytes were isolated from ovaries of PD35 mice and used for western blot. For each lane, 200 GV oocytes were used. For each experiment, at least 5 mice of each genotype were used. (B) Immunofluorescent staining of 2-month-old ovarian sections showing increased γH2AX in Ppp6c F/F ;GCre+ oocytes. Green: γH2AX; Red: MVH; Blue, DAPI. White arrows point to nucleus of control oocytes; yellow arrows point to nucleus of mutant oocytes. Bar = 20 μm. At least 3 mice of each genotype were used for analysis, and representative images are shown. (C) Decreased incidence of GVBD and PBE of Ppp6c F/F ;GCre+ oocytes. PD35 GV oocytes were isolated and matured in vitro , oocytes that resumed meiosis I (GVBD) and extruded the first polar body (PBE) were counted at 4 h and 13 h, respectively. Data are shown as mean ± SEM. *P< 0.05; **P< 0.01. Representative images of immunostaining for DNA (red) and α-tubulin (green) showing abnormal spindle assembly and aberrant chromosome alignment in Ppp6c F/F ;GCre+ oocytes at 8 h and 13 h, respectively. Bar = 10 μm. In vitro maturation experiments were repeated at least three times.

Article Snippet: Commercial antibodies were used to detect PPP6C (rabbit, A300-844A; Bethyl Laboratories, Inc.), α-tubulin (mouse, DM1A; Sigma-Aldrich), MVH (rabbit, ab13840; Abcam), γH2AX (rabbit, 9718; Cell Signaling Technology, Inc.), p-CHK1 (S345) (rabbit, BS4041; Bioworld Technology, Inc.), p-CHK2 (T68) (rabbit, BS4043; Bioworld Technology, Inc.), p-p53 (S15) (rabbit, 12571; Cell Signaling Technology, Inc.), CHK1 (rabbit, BS1052; Bioworld Technology, Inc.), p-AKT (S473) (rabbit, 4060; Cell Signaling Technology, Inc.), p-AMPK (T172) (rabbit, 2535; Cell Signaling Technology, Inc.), p-mTOR (S2448) (rabbit, 5536; Cell Signaling Technology, Inc.), p-S6K (T389) (rabbit, 9234; Cell Signaling Technology, Inc.), p-rpS6 (S240/244) (Rabbit, 5364; Cell Signaling Technology, Inc.), GAPDH (rabbit, 5174; Cell Signaling Technology, Inc.) and β-actin (mouse, sc-47778, Santa Cruz).

Techniques: Western Blot, Isolation, Staining, Mutagenesis, In Vitro, Immunostaining

(A) Western blots showing up-regulated CHK1/2-p53 pathway activity in zeocin-treated Ppp6c F/F ;GCre+ oocytes. Level of β-actin was used as internal controls. Molecular mass is given in kilodaltons. GV oocytes were isolated from ovaries of PD35 mice and treated with zeocin in vitro . For each lane, 200 GV oocytes were used. For each experiment, at least 5 mice of each genotype were used. (B) Western blots showing up-regulated CHK2-p53 pathway activity in zeocin-treated Ppp6c F/F ;GCre+ ovaries. Level of β-actin was used as internal controls. Molecular mass is given in kilodaltons. Ovary lysates were prepared from ovaries of PD35 mice after zeocin treatment in vivo . For each lane, 30 μg proteins were loaded. For each experiment, at least 3 mice of each genotype were used.

Journal: PLoS Genetics

Article Title: Protein Phosphatase 6 Protects Prophase I-Arrested Oocytes by Safeguarding Genomic Integrity

doi: 10.1371/journal.pgen.1006513

Figure Lengend Snippet: (A) Western blots showing up-regulated CHK1/2-p53 pathway activity in zeocin-treated Ppp6c F/F ;GCre+ oocytes. Level of β-actin was used as internal controls. Molecular mass is given in kilodaltons. GV oocytes were isolated from ovaries of PD35 mice and treated with zeocin in vitro . For each lane, 200 GV oocytes were used. For each experiment, at least 5 mice of each genotype were used. (B) Western blots showing up-regulated CHK2-p53 pathway activity in zeocin-treated Ppp6c F/F ;GCre+ ovaries. Level of β-actin was used as internal controls. Molecular mass is given in kilodaltons. Ovary lysates were prepared from ovaries of PD35 mice after zeocin treatment in vivo . For each lane, 30 μg proteins were loaded. For each experiment, at least 3 mice of each genotype were used.

Article Snippet: Commercial antibodies were used to detect PPP6C (rabbit, A300-844A; Bethyl Laboratories, Inc.), α-tubulin (mouse, DM1A; Sigma-Aldrich), MVH (rabbit, ab13840; Abcam), γH2AX (rabbit, 9718; Cell Signaling Technology, Inc.), p-CHK1 (S345) (rabbit, BS4041; Bioworld Technology, Inc.), p-CHK2 (T68) (rabbit, BS4043; Bioworld Technology, Inc.), p-p53 (S15) (rabbit, 12571; Cell Signaling Technology, Inc.), CHK1 (rabbit, BS1052; Bioworld Technology, Inc.), p-AKT (S473) (rabbit, 4060; Cell Signaling Technology, Inc.), p-AMPK (T172) (rabbit, 2535; Cell Signaling Technology, Inc.), p-mTOR (S2448) (rabbit, 5536; Cell Signaling Technology, Inc.), p-S6K (T389) (rabbit, 9234; Cell Signaling Technology, Inc.), p-rpS6 (S240/244) (Rabbit, 5364; Cell Signaling Technology, Inc.), GAPDH (rabbit, 5174; Cell Signaling Technology, Inc.) and β-actin (mouse, sc-47778, Santa Cruz).

Techniques: Western Blot, Activity Assay, Isolation, In Vitro, In Vivo

Journal: The EMBO Journal

Article Title: E2F1 proteolysis via SCF ‐cyclin F underlies synthetic lethality between cyclin F loss and Chk1 inhibition

doi: 10.15252/embj.2018101443

Figure Lengend Snippet:

Article Snippet: pCHK1 S317 , GeneTex , GTX22834.

Techniques: